Part:BBa_K4387001

Codon Optimized NorR

Usage and Biology

NorR is a transcriptional regulator of the nitric oxide sensitive promoter pNorV. NorR acts as an activator of pNorV in the presence of nitric oxide and represses the expression of downstream genes otherwise[1]. This regulator was codon optimized for E.coli Nissle 1917 to avoid recombination.

This part is present in the following composite parts:

• Negative Control of Nitric Oxide Sensing Genetic Circuit
• Nitric Oxide Sensing Genetic Circuit With the ETH promoter pNorV
• Nitric Oxide Sensing Genetic Circuit With One Ribosomal Binding Site
• Nitric Oxide Sensing Genetic Circuit With Two Ribosomal Binding Sites
• Nitric Oxide Sensing Genetic Circuit With Three Ribosomal Binding Sites

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

• [1] Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223